Research placement in Prof. Neva Caliskan’s laboratory at the Helmholtz Institute for RNA-based Infections Research (HIRI) - Jemma Betts

Thanks to the flexible funding I was able to undertake a 3-week research training placement in the laboratory of Prof. Neva Caliskan at the Helmholtz Institute for RNA-based Infections Research in Würzburg, Germany. The aim of this research placement was to gain specialist technical skills in optical tweezer experimental design, data acquisition and analysis. Briefly, optical tweezer experiments allow us to study the unfolding and refolding forces of single molecules by stretching and relaxing the molecule of interest. This allows us to gain insights about the stability and structure of the molecule of interest. Which complements my current PhD research investigating the mechanisms of ribosomal frameshifting during viral infections.

During my research placement I was able to gain hands on experience using the Lumicks C Trap, a novel commercial instrument. I learnt all aspects of optical tweezer experiments including RNA sample preparation, how to tether a single molecule of interest and operate the C Trap, then how to analyse data using the Practical Optical Tweezers Analysis Tool (POTATO) developed by the Caliskan laboratory. The research placement was a fantastic opportunity to learn a new, specialist biophysical technique that complements my current PhD research and was unavailable to me without undertaking this research placement. In addition, this research placement has allowed me to establish an existing international collaboration between the Hill and Caliskan laboratories and generate data that will contribute directly to my PhD project and future publications.

Without the support of the flexible funding, I would not have been able to undertake this research placement. The whole process, from writing the flexible funding grant application to working in an international research institute, has given me great confidence in my abilities. It has been a very informative placement for my career and personal development, where I have been able to experience the day-to-day workings of an international research institute. As I intend to pursue an academic career this research placement provided a unique opportunity to expand my technical skillset that I believe will enhance my employability and establish a new network of collaborators and potential future colleagues and employers.