I was awarded flexible funding to attend the Beginner Course and Advanced Course in Immunohistochemistry held at the University of Sheffield in the Sheffield Institute for Translational Neuroscience (SITraN).
My PhD project is developing and testing a new anti-virulence drug against Pseudomonas aeruginosa in cystic fibrosis patients. This project is a collaboration with an industrial partner Neem Biotech and involves testing a new anti-virulence drug in an in vivo mouse model. I wanted to learn how to stain infected and treated mouse lung samples to visualise the Pseudomonas infection in the lung. Before this course I had no experience on histology or immunohistochemistry. The Beginner course gave a general introduction into immunohistochemistry and histology as well as a practical component. This was especially useful for planning my experiments and giving me some practical experience in histology lab. The advanced course was a two-day practical which gave m experience in multiple IHC staining technique and explained why the different reagents used in the technique were chosen. I was also shown the different Image Analysis software that is available for quantifying different cell types.
By the end of the two courses, I felt confident in planning my own IHC staining protocol. The skills obtained from this course has made a massive impact on my progress and broadened my skill set as a scientist.